Efficacy of epsilon-poly-L-lysine inhibition of postharvest blue mold in apples and potential mechanisms
نویسندگان
چکیده
Penicillium expansum is a major postharvest fungal pathogen and the causal agent of blue mold decay in apples. Epsilon-poly-l-lysine (?-PL) naturally-occurring polypeptide that has strong antimicrobial activity. It primarily used to suppress foodborne pathogens bread, beverage, meat products, etc. The potential application ?-PL management diseases fruit, however, not been explored. In present study, inhibitory effect against (P. expansum) disease apples its mechanism action were investigated. Results indicated 600 mg L?1 could effectively inhibit mycelial growth P. Concentrations > 200 also inhibited germination conidia germ tube potato dextrose broth (PDB). increased with increasing concentrations concentration. Further studies possible mechanisms involved inhibition included activation defense-related enzyme activity gene expression apple fruit tissues. This polyphenol oxidase (PPO), catalase (CAT), peroxidase (POD) phenylalanine ammonialyse (PAL). stimulated production intracellular reactive oxygen species (ROS) degraded integrity cell wall plasma membrane conidia, resulting death conidial spores or their germination.
منابع مشابه
Inhibition of epsilon-poly-L-lysine biosynthesis in Streptomycetaceae bacteria by short-chain polyols.
Antimicrobial epsilon-poly-L-lysine (ePL) is secreted by Streptomycetaceae bacteria, and the mechanism of ePL biosynthesis remains to be elucidated. We previously reported that an unknown ePL derivative accumulates in the culture medium of ePL-producing bacteria when glycerol is added to the culture medium (Nishikawa and Ogawa, Appl. Environ. Microbiol. 68:3575-3581, 2002). In this study, by us...
متن کاملeffect of postharvest application of chitosan and calcium chloride on decay and quality attributes of sweet cherry
چکیده ندارد.
15 صفحه اولAntimicrobial, antioxidant, and antitumor activity of epsilon-poly-L-lysine and citral, alone or in combination
BACKGROUND Food safety is an important worldwide public health concern, and microbial contamination in foods not only leads to food deterioration and shelf life reduction but also results in economic losses and disease. OBJECTIVE The main aim of the present study was to evaluate the effect of epsilon-poly-L-lysine (ε-PL) and citral combination against Escherichia coli O157:H7 (E. coli O157:H7...
متن کاملGenome Sequence of Streptomyces albulus PD-1, a Productive Strain for Epsilon-Poly-l-Lysine and Poly-l-Diaminopropionic Acid
Streptomyces albulus PD-1, a productive strain for epsilon-poly-l-lysine and poly-l-diaminopropionic acid, was isolated from soils. We present the genome sequence of S. albulus PD-1, which may provide abundant information regarding the production of epsilon-poly-l-lysine and poly-l-diaminopropionic acid.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Postharvest Biology and Technology
سال: 2021
ISSN: ['0925-5214', '1873-2356']
DOI: https://doi.org/10.1016/j.postharvbio.2020.111346